MouSelect Protein A binding test

Purification on Protein A is the most common method for purification of antibodies, especially in the later phases of pharmaceutical development. The MouSelect kit contains the basic components needed to set up a sandwich ELISA for the assessment of Protein A binding. The components are designed to aid in an early selection of polyclonal or monoclonal antibodies, to a preferential purification on Protein A (from Staphylococcus aureus). The information of an early screening will save valuable time down-stream, since products have already been preferentially selected towards later Protein A purification. Screening on-column or in pre-filled filter plates is more time consuming and expensive, and this procedure is generally not possible for a large number of antibodies.

Key Features:

• Detection of mouse IgG1, IgG2a, IgG2b, and IgG3.
• Enough reagents included to run 8 plates, i.e. an initial screen for up to 700 samples.
• Simple operation without any sample preparation steps. Culture supernatant can generally be used without dilution.
• Possible to customize the assay to your needs.

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